To stop being gentle and start fighting hard.
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To stop being gentle and polite and start acting aggressively.
When someone takes the gloves off, they drop the careful approach and use tougher tactics.
It comes from boxing, where bare fists mean a far rougher fight.
"Play Hardball"
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To be tough from the start, not to switch from gentle to tough
"Raise the Stakes"
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To increase the risk, pressure, or importance of a situation
"Take the Gloves Off"
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To stop holding back and start being more aggressive or direct